SLC1A2 and amyotrophic lateral sclerosis: In addition, both L-glu uptake as well as EAAT2 immunoreactivity, as analyzed by Western blotting, were shown to be quantitatively reduced in postmortem tissue of ALS patients especially in the spinal cord, the tissue that is most affected by the disease (Rothstein et al., 1992; Lin et al., 1998).