AURKB and osteosarcoma: Thus, we next focused on identifying robust and reproducible cellular readouts for Aurora A and Aurora B kinase activity and employed them to systematically profile inhibitors in dose–response in three cell lines commonly used in cell biological studies: HeLa cervical carcinoma, hTERT-RPE1 retinal pigment epithelial (hereafter referred to as RPE1), and U2OS osteosarcoma cells.