This showed that Annexin V/PI fluorescence signals were increased in oocytes from HFD (14.38 ± 2.94) and ob/ob (13.19 ± 3.43) mice with obesity as compared to those in controls (1.00 ± 0.00) (P < 0.05; Fig. 4C). The gene discussed is ANXA5; the disease is obesity due to melanocortin 4 receptor deficiency.