In addition, Wongwairot et al. [33] revealed that MoAb could detect isoforms of cathepsin Ls of both juvenile and adult F. gigantica. Another study of F. gigantica reported that the diagnosis of human fasciolosis was developed by using MoAb against 49.5 kDa ES antigens of F. gigantica, but the MoAb also exhibited cross-reactivity with Schistosoma mansoni antigen [34]. This evidence concerns the gene CTSL and fascioliasis.