In the present study, we developed a MoAb-based sandwich ELISA for detection of circulating CatL1 antigen in the serum samples from mice experimentally and cattle naturally infected with F. gigantica. In addition, we were the first to develope the immunochromatographic (IC) strip test for rapid detection of fasciolosis by F. gigantica infection. This evidence concerns the gene CPT1C and fascioliasis.