Moreover, miRNA-675 was also elevated (Fig. 5c), as would be expected based on the elevation in H19. Since miRNA-675 may negatively regulate the PI3K-Akt- mTORC1 pathway, this H19/miRNA-675 elevation might contribute to mTORC1 depression in RBS. The gene discussed is AKT1; the disease is Roberts-SC phocomelia syndrome.