Thus, in comparison to the control cells, the ratio of p-Akt over Akt was markedly reduced 18.6%, 14.8%, and 72.8% and the ratio of p-mTOR over mTOR was declined 18.4%, 23.3%, and 33.1% when HT29 cells were treated with ALS at 0.1, 1, and 5 μM, respectively (p < 0.01; Figure 7A,B). This evidence concerns the gene AKT1 and amyotrophic lateral sclerosis.