The expression level of p21 Waf1/Cip1 was increased 3.3- and 2.5-fold when HT29 cells were treated with 1 and 5 μM ALS, respectively, compared to the control cells (p < 0.001; Figure 3A and Figure S3A). The gene discussed is CDKN1A; the disease is amyotrophic lateral sclerosis.