As shown in Figure 3A and Figure S3A, the expression level of p53 was increased 1.5- and 1.4-fold when HT29 cells were incubated with ALS at 1 μM (p < 0.05) and 5 μM (p > 0.05), respectively, compared to the control cells. This evidence concerns the gene TP53 and amyotrophic lateral sclerosis.