The HER2-positive BT-474 breast cancer cell line overexpressing SHP2 [19] and the MCF-10A cells and MEFs ectopically overexpressing HER2 and EGFR were used as positive controls, while parental MCF-10A cells and MEFs as well as HMLE cells were used as negative controls for overexpression. The gene discussed is PTPN11; the disease is breast cancer.