Meantime, treatment with rSjcystatin after TNBS induction also led to significant decline of the percentage of CD4+IFNγ+ in the LPMC (Fig. 4e), which was confirmed in colon homogenate supernatants by ELISA, showing a statistical I difference of IFNγ between mice with colitis (1628.6 ± 358.51) pg/ml and mice treated with TNBS-rSjcystatin (1126.8 ± 230.16) pg/ml (Fig. 6a). Here, CD4 is linked to colitis.