Compared to the control, Th1 cell populations (CD4+IFNγ+) and Th17 subsets (CD4+IL-17A+) increased in the spleen, MLN and colon tissues of mice with colitis (Fig. 4a, c) as well as the enhanced concentrations of IFNγ and IL-17 in colon homogenate supernatants (Fig. 6a). The gene discussed is CD4; the disease is colitis.