To test the effect of virus inoculum size on expression from uDNA, we reduced the virus inoculum eightfold, yielding a peak of 2 % GFP+ cells with the integration competent virus, or increased it twofold, observing little or no alteration in the kinetics of virus expression or the relative expression from the No RAL vs. +RAL infections (Additional file 1: Fig. S3A). Here, RALA is linked to infection.