PTPN6 and ovarian cancer: We confirmed these results in ovarian cancer cells and extended them to the promoter of the upstream and co-regulated gene, PTPN6. Finally, we demonstrate here that the 3D DNA loop is associated with a 2.5-fold increase in transcription read-through from the upstream gene, PTPN6. It is important to note that given the physical interaction between the two promoters that we demonstrated here, the crosslink between the promoters could also contribute to the ChIP experiments results.