PTPN6 and ovarian carcinoma: Based on standard curves obtained on serial dilutions of a bacterial artificial chromosome (BAC) DNA corresponding to the same genomic locus (Supplementary Fig. 6a), we first observed that the quantity of the intermediate transcript was equivalent to the level of the pri-PTPN6 and pri-miR-200c-141 primary transcripts in SKOV3 ovarian cancer cells at basal state (Fig. 5b).