Among these proteins, Her2 and Grb7 were positive markers of Her2 positive tumours (highly expressed); for ERPR tumours, MAPK3 (Erk1) and EEF1G were positive and negative markers, respectively; and the positive markers of TN tumours were MCM5, STMN1, GLS, RCL1, C9ORF114 and ENO1. Receiver operating characteristic curves of the classification showed an area under the curve of 0.94 for Her2 tumours, 0.87 for ERPR and 0.91 for TN (Fig. 5b). This evidence concerns the gene SPOUT1 and neoplasm.