Recently, we and others have used FRAP in more complex and physiologically relevant environments ranging from application in Drosophila (Cavey et al., 2008) to the use of E-cadherin-GFP FRAP in a mammalian system in vivo (Serrels et al., 2009), where E-cadherin-GFP-expressing squamous cell carcinoma cells were transplanted and grown subcutaneously in mice. Here, CDH1 is linked to squamous cell carcinoma.