We used reverse-phase protein arrays to look at downstream signalling to Akt, mitogen-activated protein kinase (MAPK) and S6 in the AZD8931-treated tumours and found that their activation was significantly inhibited in MMTV-NIC PTEN+/+ and MMTV-NIC PTEN+/− tumours (Fig. 1F). The gene discussed is AKT1; the disease is neoplasm.