APOE and Alzheimer disease: Similar analysis of the CA1 and CA3 areas of the hippocampus in 3xTg-AD animals revealed a 2-fold increase in treated compared with untreated animals (Fig 5A and 5B) Of note, the highest increase (5-fold) in ApoE expression was observed in the granular cell layer (GCL) of DG in the hippocampus of treated 3xTg-AD compared with untreated 3xTg-AD animals (P<0.01) (Fig 5B).