TRMT1 and leukodystrophy: We also introduced mutations in a catalytic subunit of RNAP III associated with hypomyelinating leukodystrophy (HLD) to show that a general decrease in tRNA transcription by another mechanism also increases m22G26 modification efficiency and reverses antisuppression in S. pombe. The results establish a link between RNAP III activity, tRNA production and Trm1 modification activity that impacts tRNA function.