The increased expression of cytokines Il1b, Tnfa, and Csf1, but not Il6 in this data set suggests an activation profile that is specific to prion disease and likely also unique to the in vivo environment since cocultures of microglia and neurons in the presence of PrP106−126 induces a stereotypic response with CD14-mediated detection of damaged neurons and increased expression of Il6 (96). This evidence concerns the gene IL1B and prion disease.