Additional extended immunostaining comparison of EpCAM expression illustrated in Fig. 2 showed that only colon cancer cells SW480 had strong EpCAM staining on the plasma membrane, whereas both pancreatic cancer cells PANC-1and non-small cell lung cancer (NSCLC) cells A549 only showed very weak and heterogeneous cytoplasmic and nucleus staining. This evidence concerns the gene EPCAM and malignant colon neoplasm.