In view of the extraordinary significance in terms of simultaneous phenotyping tumor biomarker protein expression and karyotyping aneuploidy of chromosome(s) in CTCs/DTCs, a novel in situ strategy of immunostaining-FISH (iFISH®) combining karyotypic CEP-FISH and phenotypic immunostaining of CD45 as well as tumor markers has been successfully developed to identify non-hematopoietic heteroploid tumor cells [7]. This evidence concerns the gene PTPRC and neoplasm.