To further investigate whether LARP1 knockdown affected the tumour initiating potential of ovarian cancer cells in vivo, we performed a limiting dilution assay, injecting decreasing numbers of SKOV3 cells with stable non-targeting (lenti-shControl) or LARP1-targeting (lenti-shLARP1) short-hairpin expression subcutaneously into NOD-SCID IL2R-gammanull (NSG) mice (Figure 5D). The gene discussed is IL2RA; the disease is ovarian cancer.