The results showed that, the microenvironment of hypoxia and vitronectin, but not other ECM, the most significantly enhanced activation of EGFRvIII and its downstream signaling effectors, i.e. ERK1/2 (MAPK), AKT and STAT3, and the expression level of the tumor cell invasion markers, i.e. matrix metalloproteinase-2 (MMP2) and MMP9 [16]. The gene discussed is STAT3; the disease is neoplasm.