To establish an LMC model with an EGFR-mutant lung cancer, we utilized the human EGFR mutant lung adenocarcinoma PC-9/ffluc (exon 19 deletion) [13], HCC827/luc, and H1975/luc cells, which were transfected with the fusion gene of luciferase for in vivo imaging. Here, EGFR is linked to lung adenocarcinoma.