To elucidate the effects of SHH inhibitors on additional TNF-α–induced inflammatory mediators, we measured Cox-2, TNF-α, iNOS, and IL-8 expression (these inflammatory mediators are all known to be involved in colitis and CAC [16]) and found their significant down-regulation in the presence of 10 mg/ml cerulenin or 30 μM cyclopamine (Figure 2A). This evidence concerns the gene SHH and colitis.