In agreement with the western blot data, real-time RT-PCR showed that co-culture with normal MSCs and CML-MSCs sharply increased Wnt signaling target genes FZD7, MDR1, Survivin, CD44, c-Myc, and Trib2 mRNA expression in K562 cells (Figure 1B). This evidence concerns the gene CD44 and chronic myelogenous leukemia, BCR-ABL1 positive.