TRS3 and INOF cells differentiated into CAF-like cells when cultured with ovarian cancer cell conditioned medium as evidenced by the induced expression of the CAF markers ACTA2, FAP, collagen 1α (COL1A1), fibronectin (FN1), and fibronectin with an alternatively spliced domain A (FN-EDA) (p < 0.05, Figure 3C and Supplementary Figure S5C). This evidence concerns the gene ACTA2 and ovarian cancer.