In conclusion, we performed a siRNA screen that led to the identification of HGS as a potential SL partner of oncogenic CTNNB1. To ensure high confidence in the hits selected with our approach, we measured three fitness variables (cell proliferation index, induction of apoptosis and number of mitotic events) [23] in two different isogenic cell line pairs from different cellular contexts (liver cancer and CRC). The gene discussed is CTNNB1; the disease is liver cancer.