As shown in Figure 4, the percentages of HepG2shMus81 and Bel‐7402shMus81 cells at S phase were increased moderately from 42.63 to 47.20% or from 23.84 to 33.96% comparing with HepG2shCtrl and Bel‐7402shCtrl cells, and the percentages at G1 phase were also decreased significantly from 53.68 to 48.70% or from 52.06 to 40.74%, suggesting that Mus81 knockdown lead to a moderate S‐phase arrest of HCC cells. The gene discussed is MUS81; the disease is hepatocellular carcinoma.