The IC50 values of EPI, 5‐FU, MMC, and cisplatin in HepG2shMus81 and Bel‐7402shMus81 cells were significantly decreased than HepG2shCtrl or Bel‐7402shCtrl cells (P < 0.05), and the reverse index (RI) value of EPI was the highest among the four chemotherapeutic drugs (Table 1), indicating that Mus81 knockdown could obviously enhance the chemosensitivity of HCC cells to common chemotherapeutic drugs, especially to EPI. This evidence concerns the gene MUS81 and hepatocellular carcinoma.