To evaluate the capacity of the tetravalent dengue vaccine to elicit T-cell responses, ED3-specific IFN-γ and IL-4 production in mice receiving three immunizations with the control vector pVax-1, pTDV-ED3, rTED3 vaccine or DNA prime-protein boost strategy using pTDV-ED3 and rTED3 (prime-boost), as shown in the top of Fig 2, were assayed by ELISPOT. This evidence concerns the gene IL4 and dengue disease.