We demonstrate that Aurora B kinase is essential to phosphorylate CHMP4C and the role of CHMP4C in radioresistance is dependent on Aurora B, which proves that CHMP4C is the major downstream target of Aurora B. Therefore, we identify the close relation between CHMP4C and Aurora B signaling pathway in mediating radiation resistance in NSCLC cells, which is independent on p53. This evidence concerns the gene TP53 and non-small cell lung carcinoma.