AFF1 and precursor B-cell acute lymphoblastic leukemia: Confirming previously published data (Guenther et al., 2008, Wilkinson et al., 2013), ChIP-seq using an MLL N-terminal antibody (Ab) (MLLN) and an AF4 C-terminal Ab (AF4C) in the MLL/AF4-positive B-ALL cell lines SEM and RS4;11 shows that MLL/AF4 binds to the BCL-2 locus (Figure 2B; Figure S2A).