To confirm a role for C/EBPβ in the development of cancer cachexia, we isolated Cebpbfl/flPax7+/+ (WT) and Cebpbfl/flPax7CreER/+ (cKO) satellite cells, and pre-treated them with CM from the PC-3 tumor or the SKOV3 tumor (control) prior to induction to differentiate in low serum conditions. The gene discussed is CEBPB; the disease is neoplasm.