To investigate the role of C/EBPβ in muscle stem cells during cancer cachexia, we used a validated tissue culture model [53] in which subconfluent C2C12 myoblasts were incubated with conditioned medium (CM) from the human prostate cancers PC-3 and DU145, or with unconditioned media (UM) for 2 days prior to induction to differentiate (Fig 2A). This evidence concerns the gene CEBPB and prostate cancer.