ELISAs revealed that the myocardial levels of IL-1β (33.2±1.6 or 21.1±0.9 pg/mg), IL-6 (68.4±2.0 or 42.3±1.7 pg/mg), and TNF-α (62.3±2.1 or 30.1±1.9 pg/mg) were significantly higher in Ad-Null mice at 1 wk or 4 wk post-MI than that of IL-1β (4.1±0.5 or 6.2±0.7 pg/mg), IL-6 (14.0±1.4 or 12.1±0.8 pg/mg), and TNF-α (9.3±0.7 or 10.1±0.6 pg/mg) in sham-operated mice. The gene discussed is TNF; the disease is myocardial infarction.