PRDM16, LMO3 and CPEB1 are hypomethylated genes in astrocytoma cells and because CPEB1 and LMO3 have been confirmed as epigenetic targets of miR-101[21], we examined the effects of miR-101 on the methylation status of PRDM16 by BSP. This evidence concerns the gene LMO3 and astrocytoma (excluding glioblastoma).