AGO2 and colonic neoplasm: To evaluate if DNA damage induced by DSBs affects the association between certain miRNAs and the RISC complex, we stimulated either TP53+/+ or TP53−/− HCT116 human colon cancer cell lines with DOX for 24 h and performed small RNA-seq from total RNA and from RNA purified from immunoprecipitated AGO2 (AGO2-RIP-seq), analyzed using a tailored bioinformatics pipeline (Supplemental Fig. S1; Supplemental Methods).