We selected a panel of 12 isogenic knockout cell lines with mutations in key oncogenic signaling pathways (Fig EV1 and Materials and Methods): three HCT116 colon cancer cell lines where the oncogenic mutation of either CTNNB1 (β‐catenin), KRAS, or PI3KCA (PI3K) was deleted, leaving only the respective wild‐type allele, as well as seven knockout cell lines for PTEN, AKT1, AKT1, and AKT2 together (AKT1/2), MAP2K1 (MEK1), MAP2K2 (MEK2), TP53, and BAX and two parental HCT116 cell lines (P1 and P2). Here, AKT2 is linked to malignant colon neoplasm.