To determine the effect of PKC isozymes on the infiltration of GBM cells, we depleted individual PKC isoforms (PKCα, PKCβ, PKCζ, and PKCδ) by siRNA in the U87 GBM and patient-derived X01 GBM cells, and analyzed the migration and invasion of GBM cells in matrigel-coated (invasion assay) or uncoated (migration assay) transwells. Here, PRKCA is linked to glioblastoma.