This result clearly supports the use of this marker together with MLH1 methylation in order to discriminate LS-MSI from S-MSI [52] as well as confirming that the concurrence of BRAF mutations with a CIMP-H phenotype is a specific feature of older onset CRC [15] but was absent in EO-MSS cancers. The gene discussed is MLH1; the disease is colorectal carcinoma.