Given that several key components of antiviral RNAi, including the Arabidopsis AGOs AGO1, AGO2, and AGO5 and rice AGO2 and AGO18, are induced during virus infection [24–27], and given that the RTL1 and RTL3 enzymes harbor RNaseIII and DRB domains that could bind to and cleave dsRNA intermediates of virus replication, we reasoned that RTL1 or RTL3 could help plants to fight against viruses if their expression is induced during virus infection and if they are expressed where viruses replicate. This evidence concerns the gene AGO2 and viral infectious disease.