In the eight human GC cell lines examined, Twist1 expression was downregulated in four cell lines (Fig 1E, left), three (KATO-III, GCIY and AGS) of which demonstrated the re-activation of Twist1 expression after treatment with 5-aza-dC by RT-PCR (example, Fig 1E, right). This evidence concerns the gene TWIST1 and gastric cancer.