Although two of the top-ranked probes (cg14868530 (annotated to CD276) and cg11998205 (annotated to TMEM143)) were significantly differentially methylated in the same direction between affected twins and their unaffected co-twin at age 5 (P = 0.035 and P = 0.036, respectively), no significant differences at age 5 were observed for the other top-ranked loci, and there was no overall correlation in within-twin differences at ages 5 and 10 for the top-ranked persistent-asthma DMPs. Here, CD276 is linked to asthma.