ERBB2 and neoplasm: Whole sections of the 54 cases with strong membranous staining by IHC were then subjected to HER2 FISH analysis; 24 of the 25 stage II–III tumours were successfully hybridized, with 20 of 24 (83.3%) showing HER2 amplification: 28 of 29 stage IV tumours were successfully hybridized, with 27 of 28 (96.4%) showing HER2 amplification (Figure 2); 10 of 53 stage IV tumours displaying weak membranous IHC staining were randomly selected for HER2 FISH analysis and none of these tumours showed HER2 amplification.