[11C]Alisertib accumulated in xenografts according to AURKA expression levels with highest albeit still modest tumor uptake and tumor-to-background ratios in A431 tumor (1.9% ± 0.2%ID/g at 25 p.i., 2.3 ± 0.8 ratio at 90 min p.i.). Here, AURKA is linked to neoplasm.