NFKB1 and neoplasm: Moreover, when cells were treated with 4T1 cell culture supernatant, p50 subunit was showed to be more phosphorylated, but no p50 phosphorylation was detected when macrophages treated with 4T1 cell culture supernatant alone, suggesting that cytokine in tumor microenvironment might play an important role together with p50 in miR-146a and miR-222 expression.