P2RX7 and neuroblastoma: Thus, treatment of serum-deprived SH-SY5Y cells with either LY29004 or API-1 for 24 h significantly reduced P2X7 transcript levels, indicating that PI3K/Akt pathway seems to have a key role in the regulation of P2X7R in neuroblastoma cells (see supplementary Fig. S1B online).