EPCAM and esophageal squamous cell carcinoma: However, when the same tissue types (ESCC or EACA) showing different degrees of differentiation were compared with EpCAM staining by SYL3C probe, a significant difference among the various differentiation stages could be observed, such that a decreasing degree of differentiation correlated with increasing expression of EpCAM among ESCC or EACA (Fig. 1A,B).