As a third model to assess whether endogenous Six1 is able to control p53, we performed KD of Six1 in the mouse mammary carcinoma 66Cl4 cell line (confirmed to contain wt p53 by sequencing) and observed that an efficient KD of Six1 leads to an increase in phospho-p53 (Supplementary Fig. 6d,e). This evidence concerns the gene TP53 and breast carcinoma.