Here, we demonstrated that TSP50-o/e cells could mainly polarize surrounding macrophages into the M2b phenotype (CCL-1high, IL-10high, CCL-5low, IL-12low and iNOSlow) in vitro, and TSP50 might drive the tumor microenvironment to a suppressive state and facilitate tumor growth by inducing macrophages to polarize into an M2 phenotype. The gene discussed is PRSS50; the disease is neoplasm.