TGFB1 and cancer: MCAS is the cancer cell line with the highest level of E-cadherin expression (Figure 4B), the marginal reduction in E-cadherin protein expression suggests that the regulation may be at the translational level, or other mechanisms such as predominant overexpression of miR200 and suppression of TGFβ signaling might be involved in spheroid formation in MCAS cancer cells (below).