MLH1 and esophageal squamous cell carcinoma: Of the18 CpG sites from MLH1 DMR region, 9 continuous clustered CpG loci (−52, −54, −64, −71, −88, −102, −111, −142, −148) were identified to be significantly hypermethylated in ESCC samples than that in NE samples (0.707±0.133 vs 0.302±0.09, p<0.001; Fig. 2A and 2B).