Next we examined the intracellular localization of these various GFP-SH2 proteins by expressing pGFP-SRC(SH2), pGFP-SHP1(NSH2), pGFP-SHP1(CSH2), pGFP-SHP2(NSH2) or pGFP-SHP2(CSH2) individually in human osteosarcoma U2OS cells, which were then plated on fibronectin-coated coverslips. The gene discussed is PTPN11; the disease is osteosarcoma.