Hepatic steatosis and the increases in the serum ALT levels were significantly severer in the eNOS-knockout mice fed an HFD than in the wild-type mice fed an HFD, while the visceral fat volume was significantly lower in the eNOS-knockout mice fed an HFD, compared with the wild-type mice fed an HFD in this study. This evidence concerns the gene GPT and Hepatic steatosis.