NOS3 and metabolic dysfunction-associated steatohepatitis: Although the present study revealed a change in the fat distribution induced by both the eNOS gene and the HFD in mice in an “early-stage NAFLD/NASH” model, longer-term analysis may detect a different phenomenon in “advanced-stage NAFLD/NASH.” A potential limitation of the current study includes the use of systemic eNOS-knockout mice.