Enhanced inhibition of p-ERK and p-AKT by AZD6244-BEZ235 compared to PLX4720-BEZ235, and to control animals treated with vehicle, was also observed in-vivo, as indicated by a reduced staining for p-ERK and p-AKT in melanoma cells from neoplastic nodules removed after the last administration of inhibitors (Figure 6A, 6B and Supplementary Figure 8 for quantitative analysis). Here, AKT1 is linked to melanoma.