BAX and melanoma: By staining Me6 melanoma cells with the 6A7 antibody, recognizing conformational changes of Bax, one of the early steps in the induction of apoptosis, we found a significantly increased fraction of Bax+ melanoma cells upon treatment for 24 h with AZD6344-BEZ235 compared to PLX4720-BEZ235 and to AZD6244-AZD8055 (Figure 9A, 9B).